![]() These findings contribute to a deeper understanding of the mechanism of action of apatinib in gastric cancer cells. Furthermore, our results suggest that the differential efficacy of apatinib in gastric cell lines may be attributed to SNW1 phosphorylation levels at a steady state. Youll need a dose response curve, from which you can calculated IC50s using a variety of free (R, NCGC CurveFit, curve-fit) or proprietrary tools (Prism. These results suggest that the mechanism of action of apatinib in KATO-III cells is independent of VEGFR2 and that the differential efficacy of apatinib was due to differences in expression patterns of receptor tyrosine kinases. Finally, we identified the molecular network related to SNW1 that was affected by treatment with apatinib. A newly developed antibody detection platform was used to perform a serial dilution enzyme-linked immunosorbent assay (ELISA) for immunoglobulin (Ig)G, IgM, and IgA SARS-CoV-2 antibodies. Furthermore, we identified SNW1 as a molecule affected by apatinib that plays an important role in cell survival. Let's see how This is a quick guide to all the steps you need to follow. Notably, KATO-III, which was the most apatinib-sensitive among the gastric cancer cell lines investigated, was the only cell line expressing c-Kit, RAF1, VEGFR1, and VEGFR3 but not VEGFR2. 17K views 2 years ago Prism Prism can easily fit a dose response curve to determine the IC50. Using integrated wet and dry approaches, we showed that apatinib was a multi-kinase inhibitor of c-Kit, RAF1, VEGFR1, VEGFR2, and VEGFR3, predominantly inhibiting c-Kit. ![]() In this study, we investigated the anti-tumor efficacy of apatinib against 13 gastric cancer cell lines and found that it differed depending on the cell line. It remains unclear why the effectivity of apatinib varies among patients and what type of patients are candidates for the treatment. ![]() In a phase III study, the objective response rate to apatinib was low. Finally, the half maximal inhibitory concentration (IC50) values were calculated from the luminous intensities using GraphPad Prism 7 (GraphPad Software Inc., Boston, MA, USA). Apatinib is known to be a highly selective vascular endothelial growth factor receptor 2 (VEGFR2) inhibitor with anti-angiogenic and anti-tumor properties.
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